Legionella : methods and protocols / edited by Carmen Buchrieser, Hubert Hilbi.
Legionnaires' disease is a severe form of pneumonia that can occur in epidemics of several hundred cases. This atypical pneumonia is characterized by a high mortality rate and affects primarily elderly and immuno-compromised individuals. An in-depth understanding of the ecology and virulence of...
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Full Text (via Springer) |
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| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York :
Humana Press : Springer,
©2013.
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 954. |
| Subjects: |
Table of Contents:
- Identification of Legionella in the environment / Natalia A. Kozak, Claressa E. Lucas, and Jonas M. Winchell
- Identification of Legionella in clinical samples / Sophie Jarraud [and others]
- Sequence-based identification of Legionella / Rodney M. Ratcliff
- Investigation of outbreaks : epidemiology / Carol Joseph
- Outbreak investigations and identification of Legionella in contaminated water / Susanne Lee and John Lee
- Typing methods for Legionella / Christian Luck [and others]
- Culturing, media, and handling of Legionella / Christa H. Chatfield and Nicholas P. Cianciotto
- Intracellular metabolism of Legionella by isotopologue profiling / Klaus Heuner and Wolfgang Eisenreich
- Induction of competence for natural transformation in Legionella pneumophila and exploitation for mutant construction / Carmen Buchrieser and Xavier Charpentier
- Constructing unmarked gene deletions in Legionella pneumophila / Andrew Bryan, Zachary D. Abbott, and Michele S. Swanson
- Natural biofilm formation with Legionella pneumophila / Emilie Portier and Yann Hechard
- Production of L. pneumophila monospecies biofilms in a low-nutrient-concentration medium / Sophie Pecastaings and Christine Roques
- Enrichment of outer membrane vesicles shed by Legionella pneumophila / Jens Jager and Michael Steinert
- Analysis of Legionella infection by flow cytometry / Andre N. Tiaden, Aline Kessler, and Hubert Hilbi
- Analysis of Legionella infection using RNAi in Drosophila cells / Dennise A. De Jesus, Tamara J. O'Connor, and Ralph R. Isberg
- Immunofluorescence imaging of Legionella infection in vitro / Stefan S. Weber and Annette Oxenius
- Electron microscopy of Legionella and Legionella-infected cells / Gary Faulkner and Rafael A. Garduno
- Pathogen vacuole purification from Legionella-infected amoeba and macrophages / Christine Hoffmann, Ivo Finsel, and Hubert Hilbi
- Methods for determining protein translocation by the Legionella pneumophila Dot/Icm type IV secretion system / Wenhan Zhu and Zhao-Qing Luo
- Subcellular localization of Legionella Dot/Icm effectors / Adam J. Vogrin [and others]
- Purification and characterization of Legionella u-box-type E3 ubiquitin ligase / Hiroki Nagai and Tomoko Kubori
- Legionella phospholipases implicated in infection : determination of enzymatic activities / Kathleen Seipel and Antje Flieger
- Interactions of Legionella effector proteins with host phosphoinositide lipids / Stephen Weber, Stephanie Dolinsky, and Hubert Hilbi
- Characterization of Legionella lipopolysaccharide / Christian Luck and Jurgen H. Helbig
- Tetrahymena and acanthamoeba model system / Sharon G. Berk and Rafael A. Garduno
- Dictyostelium host response to Legionella infection : strategies and assays / Salvatore Bozzaro, Barbara Peracino, and Ludwig Eichinger
- Caenorhabditis elegans model of Legionella infection / Ann Karen C. Brassinga and Costi D. Sifri
- Infection of nonphagocytic host cells by Legionella / Shandra R. Day, Costi D. Sifri, and Paul S. Hoffman
- Analyzing caspase-1 activation during Legionella pneumophila infection in macrophages / Christopher L. Case and Craig R. Roy
- Mouse as a model for pulmonary Legionella infection / Juliana I. Hori and Dario S. Zamboni
- Assessment of Legionella-specific immunity in mice / Stefan S. Weber, Nicole Joller, and Annette Oxenius
- Guinea pig model of Legionnaires' Disease / Paul H. Edelstein
- Human susceptibility to Legionnaires' Disease / William R. Berrington and Thomas R. Hawn
- cDNA library construction for next-generation sequencing to determine the transcriptional landscape of Legionella pneumophila / Tobias Sahr and Carmen Buchrieser
- Methods to study Legionella transcriptome in vitro and in vivo / Sebastien P. Faucher and Howard A. Shuman
- Co-immunoprecipitation : protein-RNA and protein-DNA interaction / Tobias Sahr and Carmen Buchrieser
- Identification of Legionella effectors using bioinformatic approaches / Gil Segal.