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Hematopoietic stem cell protocols / edited by Kevin D. Bunting and Cheng-Kui Qu.

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"Includes cutting-edge methods and protocols. Provides step-by-step detail essential for reproducible results. Contains key notes and implementation advice from the experts. Updating and building upon previous editions, Hematopoietic Stem Cell Protocols, Third Edition provides up-to-date protoc...

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Bibliographic Details
Online Access: Full Text (via Springer)
Other Authors: Bunting, Kevin D. (Editor), Qu, Cheng-Kui (Editor)
Format: eBook
Language:English
Published: New York : Humana Press, [2014]
Edition:Third edition.
Series:Methods in molecular biology (Clifton, N.J.) ; v. 1185.
Subjects:
Hematopoietic stem cells > Laboratory manuals.
Genetic engineering > Laboratory manuals.
Hematopoietic stem cells.
Genetic engineering.
Biology > Laboratory manuals.
Table of Contents:
  • PART I. OVERVIEW
  • 1. The Hematopoietic Stem Cell Landscape / Kevin D. Bunting and Cheng-Kui Qu.
  • PART II. STEM CELL ENRICHMENT AND HETEROGENEITY
  • 2. Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow / Huimin Cao, Brenda Williams, and Susan K. Nilsson
  • 3. Single-Cell PCR Profiling of Gene Expression in Hematopoiesis / José Teles, Tariq Enver, and Cristina Pina
  • 4. Hematopoietic Stem and Progenitor Cell Mobilization in Mice / Jonathan Hoggatt, Tiffany A. Tate, and Louis M. Pelus
  • 5. Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells / Brahmananda Reddy Chitteti and Edward F. Srour
  • 6. Flow Cytometric Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells / Lei Dong and Cheng-Kui Qu.
  • PART III. MOLECULAR AND CELLULAR CHARACTERIZATION
  • 7. Gene Expression Profiling of Hematopoietic Stem Cells (HSCs) / Nalini Raghavachari
  • 8. Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells / Wenhuo Hu and Christopher Y. Park
  • 9. DNA Methylation Profiling of Hematopoietic Stem Cells / Amber Hogart Begtrup
  • 10. Metabolic Characterization of Hematopoietic Stem Cells / Fatih Kocabas, Junke Zheng, Chengcheng Zhang, and Hesham A. Sadek
  • 11. Nanoproteomic Assays on Hematopoietic Stem Cells / Heath L. Bradley, Himalee Sabnis, Deborah Pritchett, and Kevin D. Bunting.
  • PART IV. IN VITRO ASSAYS AND DIFFERENTIATION
  • 12. Hematopoietic Differentiation of Pluripotent Stem Cells in Culture / Jason A. Mills, Prasuna Paluru, Mitchell J. Weiss, Paul Gadue, and Deborah L. French
  • 13. Ex Vivo Assays to Study Self-Renewal, Long-Term Expansion, and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells / Pallavi Sontakke, Marco Carretta, Marta Capala, Hein Schepers, and Jan Jacob Schuringa
  • 14. Ex Vivo Expansion of Murine and Human Hematopoietic Stem Cells / Phuong L. Doan and John P. Chute
  • 15. Using Microfluidics to Investigate Hematopoietic Stem Cell and Microniche Interactions at the Single Cell Level / Byungwook Ahn, Zhengqi Wang, David R. Archer, and Wilbur A. Lam.
  • PART V. TRANSPLANTATION ASSAYS AND IMAGING ENGRAFTMENT
  • 16. Five-Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells / Ryo Yamamoto, Yohei Morita, and Hiromitsu Nakauchi
  • 17. Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull / Juwell W. Wu, Judith M. Runnels, and Charles P. Lin
  • 18. Immunodeficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development / Ken-Edwin Aryee, Leonard D. Shultz, and Michael A. Brehm
  • 19. Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells / Xi C. He, Zhenrui Li, Rio Sugimura, Jason Ross, Meng Zhao, and Linheng Li.
  • PART VI. GENETIC MODIFICATION
  • 20. Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells / Marioara F. Ciuculescu, Christian Brendel, Chad E. Harris, and David A. Williams
  • 21. Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells / Niek P. van Til and Gerard Wagemaker
  • 22. High-Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies / Brian C. Beard, Jennifer E. Adair, Grant D. Trobridge, and Hans-Peter Kiem
  • 23. Barcoded Vector Libraries and Retroviral or Lentiviral Barcoding of Hematopoietic Stem Cells / Leonid V. Bystrykh, Gerald de Haan, and Evgenia Verovskaya.

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